Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
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چکیده
منابع مشابه
Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
Here we present a protocol to study intracellular protein-protein interactions that is based on the widely used biotin-avidin pull-down system. The modification presented includes the combination of this technique with cell-penetrating sequences. We propose to design cell-penetrating baits that can be incubated with living cells instead of cell lysates and therefore the interactions found will ...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2017
ISSN: 1940-087X
DOI: 10.3791/56457